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454854

Sigma-Aldrich

MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli

Synonym(e):

ERK2, MAPK2, p42 MAPK, ERK2, MAPK2, p42MAPK

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About This Item

UNSPSC-Code:
12352202

Rekombinant

expressed in E. coli

Qualitätsniveau

100

Assay

≥95% (SDS-PAGE)

Form

liquid

Spezifische Aktivität

≥5500 units/mg protein

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Fremdaktivität

Proteases, none detected

Versandbedingung

wet ice

Lagertemp.

−70°C

Allgemeine Beschreibung

Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.

Physikalische Form

In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Lew, J. 2003. Biochemistry42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Milla Koistinaho et al.
Glia, 40(2), 175-183 (2002-10-16)
Although microglial cells are thought to play a beneficial role in the regeneration and plasticity of the central nervous system (CNS), recent studies have indicated that at least some molecules released by microglia may be harmful in acute brain insults
Gary L Johnson et al.
Science (New York, N.Y.), 298(5600), 1911-1912 (2002-12-10)
Multicellular organisms have three well-characterized subfamilies of mitogen-activated protein kinases (MAPKs) that control a vast array of physiological processes. These enzymes are regulated by a characteristic phosphorelay system in which a series of three protein kinases phosphorylate and activate one
John Lew
Biochemistry, 42(4), 849-856 (2003-01-29)
Protein kinases constitute one of the largest enzyme families encoded by the human genome. Owing to their critical role in virtually all aspects of signal transduction, protein kinases have evolved stringent mechanisms for their regulation, which classically falls into two
Thomas Lanzicher et al.
Frontiers in physiology, 11, 173-173 (2020-04-08)
Titin is a large filamentous protein that forms a sarcomeric myofilament with a molecular spring region that develops force in stretched sarcomeres. The molecular spring has a complex make-up that includes the N2A element. This element largely consists of a

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