444208
MMP-1, Proenzyme, Human Rheumatoid Synovial Fibroblast
Synonym(e):
Matrix Metalloproteinase 1, Human Interstitial Collagenase, Collagenase-1
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About This Item
Empfohlene Produkte
Assay
≥90% (SDS-PAGE)
Qualitätsniveau
Form
liquid
Spezifische Aktivität
≥15 mU/mg protein
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Fremdaktivität
other MMP activity, none detected
Versandbedingung
wet ice
Lagertemp.
−70°C
Allgemeine Beschreibung
M.W. 56,000/52,000. Note: 1 mU = 1 milliunit.
Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires APMA (Cat. No. 164610; 30-60 minutes at 37°C, just prior to use) to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar Type I collagen. Must be activated just prior to use.
Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires activation by APMA (Cat. No. 164610) for 30 - 60 min. at 30°C just prior to use to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar type I collagen.
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Einheitendefinition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
Physikalische Form
In 300 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
Angaben zur Herstellung
Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Rekonstituierung
Following initial thaw, aliquot and freeze (-70°C).
Sonstige Hinweise
Liepinsh, E., et al. 2003. J. Biol. Chem.278, 25982.
Pilcher, B.K., et al. 1997. J. Cell Biol. 137, 1445.
Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
Marcy, A.I., et al. 1991. Biochemistry30, 6476.
Stricklin, et al. 1983. Biochemistry22, 61.
Pilcher, B.K., et al. 1997. J. Cell Biol. 137, 1445.
Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
Marcy, A.I., et al. 1991. Biochemistry30, 6476.
Stricklin, et al. 1983. Biochemistry22, 61.
Rechtliche Hinweise
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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