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Anti-IP₃ Receptor Mouse mAb (IPR.1)

liquid, clone IPR.1, Calbiochem®

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About This Item

UNSPSC-Code:
12352203

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

IPR.1, monoclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

rabbit, human, canine, mouse, rat, bovine, porcine

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG2b

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)

Allgemeine Beschreibung

Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.

Immunogen

a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor

Anwendung

ELISA (1:1000)

Flow Cytometry (1:500)

Immunofluorescence (1:100)

Immunohistochemistry (1:100; see application references)

Immunoprecipitation (1:100; see application references)

Radioimmunoassay (1:5000)

Immunoblotting (see application references)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Hinweis zur Analyse

Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue

Sonstige Hinweise

Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

L Cavallini et al.
The Journal of biological chemistry, 271(10), 5545-5551 (1996-03-08)
Prostaglandin I2 (PGI2) and sodium nitroprusside (SNP) induce a rapid decay of the thrombin-promoted increase of [Ca2+]i in aspirin-treated platelets incubated in the absence of external Ca2+. The mechanism of their effect was studied with a new method which utilizes
John C Hennessey et al.
Pharmacology research & perspectives, 3(2), e00112-e00112 (2015-03-03)
Endothelial cell (EC)-dependent vasodilation by proteinase-activated receptor 2 (PAR2) is preserved in small caliber arteries in disease states where vasodilation by muscarinic receptors is decreased. In this study, we identified and characterized the PAR2-mediated intracellular calcium (Ca(2+))-release mechanisms in EC
Xudong Feng et al.
Nature communications, 5, 4487-4487 (2014-07-30)
Inositol 1, 4, 5-trisphosphate receptor (IP3R)-mediated Ca(2+) release from the endoplasmic reticulum (ER) triggers many physiological responses in neurons, and when uncontrolled can cause ER stress that contributes to neurological disease. Here we show that the unfolded protein response (UPR)
L Lagostena et al.
The Journal of physiology, 531(Pt 3), 693-706 (2001-03-17)
1. Hensen's cells in the isolated cochlea were stimulated by extracellular adenosine 5'-triphosphate (ATP) applied to their endolymphatic surface while changes in membrane current and intracellular calcium concentration ([Ca2+]i) were measured simultaneously. The response consisted of (i) an initial rapid
Chenxi Guo et al.
Journal of cell science, 133(13) (2020-06-18)
The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our

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