05-596

Anti-Cdk2 Antibody, clone AN4.3

clone AN4.3, Upstate^®, from mouse

• Synonym(e): Anti-CDKN2, Anti-p33(CDK2)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: AN4.3, monoclonal
• Application: IP, WB
• Citations: 13

Eigenschaften

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: AN4.3, monoclonal
• species reactivity: mouse, Xenopus, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG

Beschreibung

General description

33kDa

Immunogen

Recombinant human cdk2

Application

0.5-2μg/ml of this lot detected cdk2 in HeLa nuclear extract. 4μg of a previous lot immunoprecipitated cdk2 from 500μg of mouse 3T3 nuclear extract.

Detect Cdk2 with Anti-Cdk2 Antibody, clone AN4.3 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

cdk2, does not react with other cdks

Physical form

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, and 30% glycerol.

Format: Purified

Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

routinely evaluated by immunoblot on HeLa nuclear extract

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1