Ni-NTA functionalized gold nanoparticles are suitable for binding of proteins with a C-, or N-terminal 6X His tag. These gold nanoparticles allow for rapid generation of gold conjugates for subsequent use in downstream assays. Ni-NTA functionalized gold nanoparticles are more than 95% spherical and have uniform size distribution (CV <12%).
Anwendung
Ideal for development of gold conjugates for use in applications such as blotting, lateral flow assays, microscopy, and transmission electron microscopy (TEM).
Lagerung und Haltbarkeit
When stored for a long period of time gold nanoparticles may sediment at the bottom of the vial, which is especially prominent for larger particle sizes. Prior to use, re-suspend the sedimented particles by thorough mixing until a homogenous solution is obtained.
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