Salmonella Detection Procedure (EN ISO 6579-1)

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food production chain.

Like the preceding ISO 6579:2002+Amd 1:2007, the latest ISO 6579-1:2017 covers the testing of products intended for human consumption, animal feeding and environmental samples in food production and handling.

In addition, EN ISO 6579-1:2017 now includes testing of milk and milk products as well as samples from the primary production stage, such as animal feces, dust and boot socks.

• Procedure for Salmonella Detection According to EN ISO 6579-1
• EN ISO 6579-1 At a Glance
• Compliance to EN ISO 6579-1
• Materials

Procedure for Salmonella detection according to EN ISO 6579-1

Day	Procedure Step
Day 1	Pre-enrichment	Sample + Buffered Peptone Water (BPW) Tenfold dilution 18 h (± 2) • 34 °C - 38 °C
↓
Day 2	Selective enrichment	0.1 mL BPW to 10 mL RVS broth or MSRV agar 24 h (± 3) • 41.5 °C (± 1)   1 mL BPW to 10 mL MKTTn 24 h (± 3) • 37 °C (± 1)
↓
Day 3	Plating-out	XLD agar 24 h (± 3) • 37 °C (± 1)   +   Second isolation agar
↓		Growth Interpretation
Day 4, 5, 6	Confirmation ↓ Expression of result	Test at least 1 typical or suspect colony. If negative, test additional 4 marked colonies. Non-selective agar (e.g. Nutrient Agar) 24 h (± 3) • 34 °C - 38 °C Biochemical/serological testing Expression of results

EN ISO 6579-1 at a Glance

• Part one (detection) of three-part EN ISO 6579 replaces EN ISO 6579:2004+Amd 1:2007
• EN ISO 6785:2007 (dairy products) method details now incorporated
• Samples from primary production stage added to scope
• Incubation temperature range for non-selective media extended to 34 – 38 °C
• Choice of RVS or MSRV agar for selective enrichment of food, animal feed and environmental samples
• Directions for choice of second isolation medium in addition to XLD agar
• Only one suspect colony needed for confirmation (if negative, 4 more to be tested)
• Optional: biochemical confirmation on well-isolated colony direct from selective plate
• β-galactosidase and indole tests now optional, Voges-Proskauer reaction omitted
• Detection of Salmonella Typhi and S. Paratyphi described 
• Performance testing for the culture media in-depth  

As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages and water microbiology, we closely track and supports the development of relevant standards aimed at increasing consumer confidence and safety.

Our GranuCult^® granulated media, Readybag^® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ & ReadyTube^® ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.  

Compliance to EN ISO 6579-1

We have implemented all the requirements described in EN ISO 6579-1. The following culture media and accessories described in ISO 6579-1 are available:  

Product	Pack Size	Catalog No.	Other Pack Sizes Available
GranuCult® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP	500 g	1.07228.0500	5 kg, 25 kg
Readybag® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP, 5.7 g, irradiated	60 bags	1.00908	60 bags x 29 g,
			35 bags x 86 g
ReadyTube® 225 BPW ISO 6579, ISO 6887, ISO 21528, ISO 11290, FDA-BAM and EP	6 x 225 mL	1.46404.0006	6 x 1000 mL,
			20 x 9 mL
GranuCult® RVS (RAPPAPORT-VASSILIADIS-Soya) Broth (Base) acc. ISO 6579	500 g	1.07700.0500
Novobiocin Sodium Salt	1 g	N6160-1-G	5 g, 25 g
ReadyTube® 10 RVS Broth ISO 6579	20 x 10 mL	1.46694.0020
GranuCult® MKTTn (MULLER KAUFFMANN Tetrathionate Novobiocin) Broth (Base) acc. ISO 6579	500 g	1.05878.0500
Potassium Iodide	250 g	1.05043.0250
Iodine resublimed	100 g	1.04761.0100
MSRV (Modified Semi-solid RAPPAPORT-VASSILIADIS) Medium (Base) acc. ISO 6579	500 g	1.09878.0500
MSRV Selective Supplement	10 x 1 vial	1.09874.0010
ReadyTube® 12 MSRV Medium ISO 6579	100 x 12 mL	1.46622.0100
GranuCult® XLD (Xylose Lysine Deoxycholate) agar acc. ISO 6579	500 g	1.05287.0500
ReadyPlate™ XLD Agar ISO 6579	20 x 90 mm	1.46751.0020
RAMBACH® Agar	4 x 250 mL	1.07500.0001	4 x 1000 mL, 1 x 50 L
RAMBACH® Agar ready-to-use	20 x 90 mm	1.46719.0020	100 x 90 mm
GranuCult® Nutrient agar acc. ISO 6579, ISO 10273 and ISO 21528	500 g	1.05450.0500
Bismuth Sulfite Agar acc. WILSON-BLAIR	500 g	1.05418.0500
Triple Sugar Iron Agar	500 g	1.03915.0500
Urea Agar (Base) acc. CHRISTIANSEN	500 g	1.08492.0500
Urea GR for Analysis ACS, Reagent Ph Eur	500 g	1.08487.0500
MR-VP (Methyl Red-VOGES-PROSKAUER) Broth	500 g	1.05712.0500
KOVAC’S Indole Reagent	100 mL	1.09293.0100

Materials