Accéder au contenu
Merck

Protective effects of glucosamine hydrochloride against free radical-induced erythrocytes damage.

Environmental toxicology and pharmacology (2014-06-25)
Khadijeh Jamialahmadi, Omid Arasteh, Maryam Matbou Riahi, Soghra Mehri, Bamdad Riahi-Zanjani, Gholamreza Karimi
RÉSUMÉ

Glucosamine (GlcN) is an important precursor in the biochemical synthesis of glycosylated proteins and lipids in human body. It gains importance because of its contribution to human health and its multiple biological and therapeutic effects. In this study, the in vitro oxidative hemolysis of rat erythrocyte was used as a model to study the potential protective effect of glucosamine hydrochloride against free radical-induced damage of biological membranes. Glucosamine hydrochloride exhibited dose-dependent DPPH antioxidant activity. Oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by a water-soluble free radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) were significantly suppressed by GlcN in a time and dose dependent manner. GlcN also prevented the depletion of cytosolic antioxidant glutathione (GSH) in erythrocytes. These results indicated that glucosamine hydrochloride efficiently protected erythrocytes against free radicals and it could be recommended as a pharmaceutical supplement to alleviate oxidative stress.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Acide trichloroacétique solution, 6.1 N
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
D-(+)-Glucosamine hydrochloride, ≥99% (HPLC), powder
Sigma-Aldrich
Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
Sigma-Aldrich
2-Thiobarbituric acid, ≥98%
Sigma-Aldrich
Trichloroacetic acid, BioUltra, ≥99.5% (T)
USP
Glucosamine hydrochloride, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
D-(+)-Glucosamine hydrochloride, ≥99%, BioReagent, suitable for cell culture
Sigma-Aldrich
Trichloroacetic acid, BioXtra, ≥99.0%
Sigma-Aldrich
Trichloroacetic acid, ≥99.0% (titration)
Supelco
Glucosamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
Glucosamine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Glucosamine for system suitability, European Pharmacopoeia (EP) Reference Standard