SRP5335

PP2C-α, active, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : FLJ42306, MGC9201, PP2C-ALPHA, PP2CA, PPM1A
• Numéro CAS: 362690-38-8
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 960-1440 nmol/min·mg
• Poids mol.: ~105 kDa
• Numéro d'accès NCBI: NM_021003
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PPM1A(5494)

Description

Description générale

Protein phosphatase 2C-α (PP2C-α), also called protein phosphatase, Mg²⁺/Mn²⁺ dependent 1A (PPM1A), is a member of the family of Ser/Thr protein phosphatases and is composed of a catalytic subunit and a constant regulatory subunit. PP2C-α is highly expressed in heart and skeletal muscle. The gene encoding this protein is localized on human chromosome 4q12.

Actions biochimiques/physiologiques

Protein phosphatase 2C-α (PP2C-α) or PPM1A is known to be negative regulators of cell stress response pathways. It has been reported to negatively regulate TGF-β signaling pathway and cell cycle. PP2C-α has been shown to dephosphorylate cyclin-dependent kinases (CDKs) and it may be involved in cell cycle control. It modulates cell migration and invasion. Overexpression of PP2C-α is reported to cause cell-growth arrest or cell death.

Forme physique

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Notes préparatoires

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable