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Key Documents

SRP3102

Sigma-Aldrich

Klotho human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonyme(s) :

KLA

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in CHO cells

Pureté

≥98% (HPLC)
≥98% (SDS-PAGE)

Forme

lyophilized

Activité spécifique

0.5—2.0 μg/mL

Poids mol.

65-70 kDa

Conditionnement

pkg of 20 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Couleur

white to off-white

Adéquation

suitable for molecular biology

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... KL(9365)

Description générale

Research area: Cancer

Human Klotho exists in both membrane-bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. The full-length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Recombinant human Klotho is a 65-70kDa glycoprotein containing 516 amino acid residues.

Application

Klotho human has been used to study the role of klotho in liver cancer.It has also been used in qualitative and quantitative analysis of Klotho protein by immunoblot and enzyme-linked immunosorbent assay (ELISA) tests respectively, in the heart tissue and coronary effluents to test the contribution of Klotho protein in cardioprotection during ischemia/reperfusion injury.

Actions biochimiques/physiologiques

Klotho (KL) is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of klotho, the function of FGF-23 is abolished. Klotho might play a protective role in type 1 diabetes mellitus against atherosclerosis and endothelial dysfunction. Absence of klotho in mice causes age-associated traits, such as short lifespan, atherosclerosis, pulmonary emphysema, and osteoporosis. It is also involved in cervical, lung, and breast cancer. In follicular thyroid cancer, it suppresses growth and enhances cell death by controlling the expression of stanniocalcin-1.

Séquence

EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH

Forme physique

Lyophilized from 15 mM Sodium Phosphate pH 7.3.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Klotho up-regulates renal calcium channel transient receptor potential vanilloid 5 (TRPV5) by intra- and extracellular N-glycosylation-dependent mechanisms.
Wolf MT, et al.
The Journal of Biological Chemistry, 289, 35849-35857 (2014)
Membrane-bound Klotho is not expressed endogenously in healthy or uraemic human vascular tissue.
Mencke R, et al.
Cardiovascular Research, 108, 220-231 (2015)
Klotho inhibits human follicular thyroid cancer cell growth and promotes apoptosis through regulation of the expression of stanniocalcin-1.
Dai D, et al.
Oncology Reports, 35, 552-558 (2016)
The beta-glucuronidase klotho exclusively activates the epithelial Ca2+ channels TRPV5 and TRPV6.
Lu P, et al.
Nephrology, Dialysis, and Transplantation, 23, 3397-3402 (2008)
Seok-Jo Kim et al.
American journal of physiology. Lung cellular and molecular physiology, 313(1), L16-L26 (2017-04-22)
Alveolar epithelial cell (AEC) apoptosis and inadequate repair resulting from "exaggerated" lung aging and mitochondrial dysfunction are critical determinants promoting lung fibrosis. α-Klotho, which is an antiaging molecule that is expressed predominantly in the kidney and secreted in the blood

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