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Key Documents

SRP3003

Sigma-Aldrich

Activin A human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

Synonyme(s) :

Activin beta-A, FRP, Inhibin beta-1

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥97% (HPLC)
≥97% (SDS-PAGE)

Forme

lyophilized

Puissance

≤2.0 ng/mL ED50

Poids mol.

26.0 kDa

Conditionnement

pkg of 10 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<0.1 EU/μg endotoxin, tested

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... INHBA(3624)
mouse ... Inhba(16323)
rat ... Inhba(29200)

Description générale

Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α. Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.

Application

Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.
Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.

Actions biochimiques/physiologiques

Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects. In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.

Séquence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Forme physique

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Generation of an induced pluripotent stem cell line from chorionic villi of a Turner syndrome spontaneous abortion.
Parveen S
Stem Cell Research, 12-16 (2017)
Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y and Massague J
Cell, 113, 685-700 (2003)
Activin A is associated with asthma in underweight and overweight patients.
Zhang LL and Liu CT
Genetics and molecular research : GMR, 14, 440-452 (2015)
The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.
Woolnough JL, et al.
PLoS ONE, 11, e0157276-e0157276 (2016)
Serum and tissue expression of activin a in postmenopausal women with breast cancer.
Reis FM, et al.
The Journal of Clinical Endocrinology and Metabolism, 87, 2277-2282 (2002)

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