SMB00704

Lipopolysaccharides from Proteus mirabilis

purified by phenol extraction

• Synonyme(s) : LPS
• Numéro CE : 297-473-0
• Code UNSPSC : 12352211
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: bacterial (Proteus mirabilis)
• Niveau de qualité: 200
• Forme: lyophilized powder
• Produit purifié par: phenol extraction
• Impuretés: <3.00% protein (Lowry-TCA)
• Couleur: white to faint yellow
• Solubilité: water: 0.90-1.10 mg/mL, slightly hazy to hazy, colorless to light yellow
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Lipopolysaccharides (LPS′s) are complex glycolipids that are characteristic components of the cell wall of Gram-negative bacteria. They consist of a lipid A moiety linked to an antigenic O-polysaccharide.
Proteus mirabilis is a Gram-negative facultative anaerobic rod-shaped bacterium. Bacteria of the genus Proteus of the family Enterobacteriaceae are opportunistic human pathogens responsible for wound and burn infections as well as skin, eye, ear, nose, throat, urinary tract, and gastrointestinal infections and bacteremias.

Application

• Lipopolysaccharide structure modulates cationic biocide susceptibility and crystalline biofilm formation in Proteus mirabilis.: The study explores how variations in lipopolysaccharide structure affect the susceptibility of Proteus mirabilis to biocides and its ability to form crystalline biofilms, informing strategies to combat biofilm-associated infections (Clarke et al., 2023).

Actions biochimiques/physiologiques

Lipopolysaccharide (LPS) and its lipid A moiety stimulate cells of the innate immune system by the toll-like receptor 4 (TLR4), a member of the toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs). Lipid A plays a role in the biological properties attributed to endotoxic LPS. LPS is a highly immunogenic antigen that has the ability to enhance immune responses.The outer-membrane lipopolysaccharide is considered an important virulence factor of Proteus. It has been suggested that the immunological response against P. mirabilis, LPS might play a role in rheumatoid arthritis. A possible correlation between the abundance of P. mirabilis in the intestine and obesity was suggested recently. The purified LPS from P. mirabilis OXK is used in the Weil-Felix test for the diagnosis of rickettsiosis (scrub typhus) caused by the bacterium Orientia (Rickettsia) tsutsugamushi.

Notes préparatoires

Soluble in water and cell culture medium DMEM (1 mg/mL) yielding a clear to slightly hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable