P7899
Anti-Peroxidase antibody produced in rabbit
fractionated antiserum, lyophilized powder
Synonyme(s) :
Rabbit Anti HRP
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
fractionated antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized powder
Espèces réactives
horseradish
Technique(s)
indirect ELISA: 1:100,000-1:200,000
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Immunogène
Purified peroxidase from horseradish
Application
Anti-Peroxidase antibody produced in rabbit has been used in:
- immunoblot assays
- enzyme-linked-immunosorbent assay (ELISA)
- immunoprecipitation
Actions biochimiques/physiologiques
Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.
Forme physique
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Reconstitution
Reconstitute with deionized water.
Remarque sur l'analyse
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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