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P3889

Sigma-Aldrich

Polygalacturonic acid

≥85% (titration), from oranges

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About This Item

Formule linéaire :
(C6H8O6)n
Numéro CAS:
25990-10-7
Numéro MDL:
MFCD00131972
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Source biologique

oranges

Niveau de qualité

200

Pureté

≥85% (titration)

Forme

powder

Couleur

white to brown

Pf

60 °C

Solubilité

aqueous NaOH: 1%, clear to hazy

Température de stockage

room temp

InChI

1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1

Clé InChI

AEMOLEFTQBMNLQ-BKBMJHBISA-N

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Catégories apparentées

Description générale

Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.

Application

Polygalacturonic acid has been used:
  • in the preabsorption of 2F4 primary antibody solution
  • as a substrate to determine polygalacturonase activity
  • in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases

Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca2+ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.

Actions biochimiques/physiologiques

In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.

Autres remarques

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Marianne Hiorth et al.
International journal of pharmaceutics, 308(1-2), 25-32 (2005-12-06)
This study has investigated the potential of immersion coating calcium containing pellet cores first with pectin, and then with two different cross-linkers, calcium or chitosan. The interaction between pectin and calcium, and between pectin and chitosan, are believed to slow
Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan
Bonnin E, et al.
Carbohydrate Polymers, 74(3), 411-418 (2008)
Kun Cheng et al.
Drug development and industrial pharmacy, 30(4), 359-367 (2004-05-11)
Insulin-loaded calcium pectinate nanoparticles were prepared as a potential colonic delivery system by ionotropic gelation with calcium ions. The effects of pectin molecular weight (Mv) and formulation pH on the characteristics of the nanoparticles were evaluated. Commercial pectins, LM101 and
Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan
E. Bonnin.
Carbohydrate Polymers, 74, 411-418 (2008)
Bacterial Wilt Disease: Molecular and Ecological Aspects, 171-171 (1998)
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Protocoles

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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