P354-05
Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
Produits recommandés
Source biologique
Porcine aorta (normal)
Niveau de qualité
Forme
solid
Conditionnement
pkg of 500,000 cells
Fabricant/nom de marque
Cell Applications, Inc
Mode de croissance
Adherent
Caryotype
2n = 38
Morphologie
smooth muscle
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
cardiovascular diseases
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
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PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Origine de la lignée cellulaire
Aorta
Application
aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation
Composants
Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
Notes préparatoires
- 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Procédure de repiquage
Please refer to the PAOSMC Culture Protocol.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Nina Parmar et al.
Tissue engineering. Part C, Methods, 21(4), 404-412 (2014-09-11)
Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are
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