Protease from Bacillus sp.

Name: Protease from <I>Bacillus</I> sp.
Brand: SIGMA

liquid, ≥16 U/g

Synonyme(s) :

Savinase^® 16.0L

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A propos de cet article

UNSPSC Code:

12352204

EC Number:

232-752-2

NACRES:

NA.54

MDL number:

MFCD00132092

Specific activity:

≥16 U/g

Biological source:

Bacillus sp.

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Propriétés

biological source

Bacillus sp.

Quality Segment

200

form

liquid

specific activity

≥16 U/g

mol wt

20-30 kDa

storage temp.

2-8°C

Description

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Product P3111 is secreted from Bacillus sp.. It is a serine-type protease characterized by excellent performance at elevated temperature and pH. Product P3111 is extremely stable under harsh storage conditions, such as in the presence of sodium hypochlorite. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typically stabilized by Ca²⁺ and have high isoelectric points. It is highly soluble in detergents at a wide range of pH and temperatures.