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Key Documents

M7769

Sigma-Aldrich

IgG2a, Kappa from murine myeloma

clone UPC 10, ascites fluid, lyophilized powder

Synonyme(s) :

Mouse IgG2a-κ

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Clone

UPC 10, monoclonal

Forme

lyophilized powder

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. The hapten binding specificity of the UPC-10 line is for β-2-6-linked fructosan. The ascites produced from the UPC-10 tumor line contains IgG2a, K in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration. IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

Spécificité

Antigen Specificity: β-2,6-fructosan

Trace amounts of lambda chain may be present.

Immunogène

The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.

Application

IgG2a, Kappa from murine myeloma has been used in immunofluorescence and immunohistochemistry.

Actions biochimiques/physiologiques

The IgG2a (immunoglobulin G 2a) plays a vital role in stimulating autoimmunity in mice. This gene is expressed at high level during virus infection. IgG2a offers host defence through activation of complement system. This antibody has specificity towards Fc receptors expressed on phagocytes and stimulates antibody-dependent cell mediated cytotoxicity. IgG2a also induces macrophage-mediated opsonophagocytosis, which has been observed in response to influenza virus and leads to its clearance from the infected cells.

Forme physique

The product provided is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, with no preservatives added.

Notes préparatoires

Storage and Stability: Store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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The Journal of biological chemistry, 272(44), 27830-27838 (1997-11-05)
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O Tabary et al.
Journal of immunology (Baltimore, Md. : 1950), 164(6), 3377-3384 (2000-03-08)
Increasing evidence suggests that in airways from cystic fibrosis (CF) patients, inflammation may precede bacterial infection and be related to an endogenous dysregulation of proinflammatory cytokines in airway epithelial cells. Several investigators have reported that, in CF airway fluids, elevated
Three classes and four (sub) classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Bazin H, et al.
European Journal of Immunology, 4(1), 44-48 (1974)
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Matsui M S, et al.
The Journal of Investigative Dermatology. Symposium Proceedings / The Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, 14(1), 56-59 (2009)

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