M3913
SigmaMarker™
low range, mol wt 6,500-66,000 Da
Synonyme(s) :
protein markers, protein standards
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A propos de cet article
Form:
lyophilized powder
Storage temp.:
2-8°C
Service technique
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form
lyophilized powder
usage
sufficient for 10-20 large gels (16 × 18 cm), sufficient for 20-30 mini-gels (10 × 10 cm), vial sufficient for 50-100 PhastGels
mol wt
6,500-66,000 Da
storage temp.
2-8°C
Application
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Preparation Note
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
Other Notes
Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Will yield eight protein bands with Coomassie Blue staining.
Legal Information
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Sang Jun Lee et al.
Applied and environmental microbiology, 68(8), 3919-3924 (2002-07-31)
Gram-negative bacteria can communicate with each other by N-acyl homoserine lactones (AHLs), which are quorum-sensing autoinducers. Recently, the aiiA gene (encoding an enzyme catalyzing the degradation of AHL) has been cloned from Bacillus sp. strain 240B1. During investigations in the
Antonios Matsakas et al.
Experimental physiology, 91(6), 983-994 (2006-07-29)
The aim of this study was to test the hypothesis that swimming training might impact differentially myostatin expression in skeletal muscles, depending on fibre type composition, and in cardiac muscle of rats. Myostatin expression was analysed by real time reverse
Andrés González et al.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 46(5), 493-501 (2018-08-02)
Recombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium Anabaena sp. PCC 7120 as a laboratory tool
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| M3913-10VL | 04061838256065 |
| M3913-1VL | 04061835490141 |