L9267
Lectin from Lens culinaris (lentil)
lyophilized powder
Synonyme(s) :
Lens culinaris agglutinin, LcH
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Forme
lyophilized powder
Niveau de qualité
Puissance
<16 μg per mL agglutination activity
Impuretés
salt, free
Température de stockage
2-8°C
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Description générale
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Application
Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).
Actions biochimiques/physiologiques
LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.
Qualité
Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.
Notes préparatoires
Highly purified by affinity chromatography.
Remarque sur l'analyse
Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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