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L5418

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

Ready Made solution, 1 mg/mL

Synonyme(s) :

LPS

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About This Item

Numéro MDL:
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Source biologique

Escherichia coli (O55:B5)

Niveau de qualité

Forme

aqueous solution

Concentration

1 mg/mL

Couleur

white to faint yellow

Solubilité

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Description générale

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O55:B5. The source strain is CDC 1644-70. This LPS serotype has been used in studies of septic shock and to induce myocarditis.

Application

Les lipopolysaccharides (LPS) sont des constituants caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné via le récepteur TLR4 (Toll-Like Receptor 4), un membre de la famille des récepteurs de type Toll, qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid
Janny C de Grauw et al.
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Inflammation is an important feature of many joint diseases, and levels of cartilage biomarkers measured in synovial fluid may be influenced by local inflammatory status. Little is known about the magnitude and time course of inflammation-induced changes in cartilage tissue
Long-Xiang Su et al.
Chinese medical journal, 132(11), 1336-1343 (2019-03-19)
It has recently been recognized that serum vimentin is elevated in infectious diseases, and that vimentin plays a role in regulating neutrophils and macrophages associated inflammation. However, the mechanisms are unclear. This study was designed to explore the role of

Articles

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Contenu apparenté

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

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