L4418

Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Amine oxidase, flavin-containing, 2, Anti-BRAF-HDAC complex protein 110, Anti-KIAA0601 protein, Anti-Lysine-specific demethylase 1, Anti-NPAO, Anti-Nuclear Polyamine Oxidase
• Numéro MDL: MFCD09039194
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 110-115 kDa
• Espèces réactives: human, mouse (predicted), rat (predicted)
• Concentration: ~1 mg/mL
• Technique(s): immunocytochemistry: 5-10 μg/mL using MCF7 cells fixed with formaldehyde; immunoprecipitation (IP): 1-2 μg using MCF7 cell lysates; western blot: 1-2 μg/mL using MCF7 cell lysates (2 bands, 2 isoforms)
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AOF2(23028) ; mouse ... Aof2(99982)

Description

Description générale

Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin containing domain 2 (AOF2) is a lysine specific demethylase. It is a nuclear protein containing a Swi3p, Rsc8p and Moira (SWIRM) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain.

Immunogène

synthetic peptide corresponding to amino acids 835-852 of human LSD1 (AOF2) (isoform b), conjugated to KLH via an N-terminal added cysteine reisidue. The immunizing sequence is present also in isoform a. This sequence is conserved in human, rat, and mouse.

Application

Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit immunoblotting, immunoprecipitation and immunofluorescence.

Actions biochimiques/physiologiques

Lysine specific demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription. LSD1 (AOF2) action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 (AOF2) leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to de-repression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors. REST corepressor 1 (CoREST), a transcriptional corepressor, promotes demethylation by enhancing the association of LSD1 (AOF2) with the nucleosomes, and it also protects LSD1 (AOF2) from proteasomal degradation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable