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Key Documents

F9679

Sigma-Aldrich

Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-FAS, Anti-FASN

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 270 kDa

Espèces réactives

mouse, rat, human

Concentration

1 mg/mL

Technique(s)

western blot: 0.5-1 μg/mL using lysates of Rat1 cells
western blot: 1-2 μg/mL using lysates of human HEK 293-T cells

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... FASN(2194)
mouse ... Fasn(14104)
rat ... Fasn(50671)

Description générale

Fatty acid synthase (FAS, FASN) is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).

Immunogène

synthetic peptide corresponding to amino acids 2497-2511 of human fatty acid synthase, conjugated to KLH via an N-terminal added lysine residue. The corresponding sequence is conserved in human, rat, and mouse.

Application

Anti-Fatty Acid Synthase (C-Terminal) antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of Rat1 cells and 1-2μg/mL using lysates of human HEK 293-T cells.

Actions biochimiques/physiologiques

FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Fatty acid synthase, a proficient multifunctional enzyme.
S J Wakil
Biochemistry, 28(11), 4523-4530 (1989-05-30)
Ellen De Schrijver et al.
Cancer research, 63(13), 3799-3804 (2003-07-04)
Fatty acid synthase (FASE), a key enzyme in the biosynthesis of fatty acids, is markedly overexpressed in many human epithelial cancers, rendering it an interesting target for antineoplastic therapy. Here, using the potent and highly sequence-specific mechanism of RNA interference
Subrahmanyam S Chirala et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(11), 6358-6363 (2003-05-10)
In animals, including humans, the source of long-chain saturated fatty acids is de novo synthesis, which is mediated by fatty acid synthase (FAS), ingested food, or both. To understand the importance of de novo fatty acid synthesis, we generated FAS
A Jayakumar et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(19), 8695-8699 (1995-09-12)
Fatty acid synthase (FAS; EC 2.3.1.85) was purified to near homogeneity from a human hepatoma cell line, HepG2. The HepG2 FAS has a specific activity of 600 nmol of NADPH oxidized per min per mg, which is about half that
Sucharita Bandyopadhyay et al.
Oncogene, 24(34), 5389-5395 (2005-05-18)
Fatty acid synthase (FAS), a key enzyme of the fatty acid biosynthetic pathway, has been shown to be overexpressed in various types of human cancer and is, therefore, considered to be an attractive target for anticancer therapy. However, the exact

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