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D6046

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate, sodium pyruvate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose is a fully supplemented liquid form of the original Dulbecco′s formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
Dulbecco′s Modified Eagle′s Medium - low glucose has been used in the maintenance/culturing of mammalian cells.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose.
Anthonisen EH et al.
The Journal of Biological Chemistry, 285, 1607-1607 (2010)
Huong Thi Pham et al.
PLoS genetics, 14(8), e1007574-e1007574 (2018-08-04)
The broadly conserved bacterial signalling molecule cyclic-di-adenosine monophosphate (c-di-AMP) controls osmoresistance via its regulation of potassium (K+) and compatible solute uptake. High levels of c-di-AMP resulting from inactivation of c-di-AMP phosphodiesterase activity leads to poor growth of bacteria under high
Nicotine increases cancer stem cell population in MCF-7 cells.
Hirata N et al.
Biochemical and Biophysical Research Communications, 403, 138-138 (2010)
High glucose-induced mitochondrial respiration and reactive oxygen species in mouse cerebral pericytes is reversed by pharmacological inhibition of mitochondrial carbonic anhydrases: Implications for cerebral microvascular disease in diabetes.
Shah GN et al.
Biochemical and Biophysical Research Communications, 440, 354-354 (2013)
June Guo et al.
Metabolism: clinical and experimental, 68, 108-118 (2017-02-12)
Our laboratory has shown that insulin's effect to decrease neointimal thickness after arterial injury is greatly diminished in insulin resistant conditions. Thus, in these conditions, a better alternative to insulin could be to use an insulin sensitizing agent. Metformin, the

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