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Merck

D5523

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

DME, DMEM

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Service technique
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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Preparation Note

Formulated to contain 10.0 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.


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Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Akon Higuchi et al.
Scientific reports, 5, 10217-10217 (2015-05-15)
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs
Gayatri Jamwal et al.
Biochimica et biophysica acta, 1865(6), 920-931 (2018-04-06)
IGF1R is a ubiquitous receptor tyrosine kinase that plays critical roles in cell proliferation, growth and survival. Clinical studies have demonstrated upregulation of IGF1R mediated signaling in a number of malignancies including colon, breast, and lung cancers. Overexpression of the
Zikai Feng et al.
Pharmaceuticals (Basel, Switzerland), 13(2) (2020-02-16)
Short-chain quinones (SCQs) have been identified as potential drug candidates against mitochondrial dysfunction, which is largely dependent on their reversible redox characteristics of the active quinone core. We recently synthesized a SCQ library of > 148 naphthoquinone derivatives and identified



Numéro d'article de commerce international

RéférenceGTIN
D5523-50L04061833586365
D5523-50K04061834180289
D5523-BIBC04061826573594
D5523-10L04061833586358
D5523-10X1L04061835522576