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D1319

Sigma-Aldrich

Anti-DAP Kinase 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-DAPK, Anti-DAPK1, Anti-Death associated protein kinase 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~160 kDa

Espèces réactives

mouse (predicted), human, rat (predicted)

Concentration

~1 mg/mL

Technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells
western blot: 1-2 μg/mL using whole extracts of human HeLa cells

Numéro d'accès UniProt

P53355

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DAPK1(1612)
mouse ... Dapk1(69635)
rat ... Dapk1(306722)

Catégories apparentées

Description générale

DAP-kinase 1 is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains, one being Ca2+/calmodulin dependent.

Spécificité

Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide.

Immunogène

synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells).
Anti-DAP Kinase 1 antibody produced in rabbit has been used in immunoprecipitation and western blotting.

Actions biochimiques/physiologiques

Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Grifolin, a potent antitumour natural product upregulates death-associated protein kinase 1 DAPK1 via p53 in nasopharyngeal carcinoma cells
Luo XJ, et al.
European Journal of Cancer, 47(2), 316-325 (2011)
Yao Lin et al.
The FEBS journal, 277(1), 48-57 (2009-11-03)
Death-associated protein kinase (DAPK) is a stress-regulated protein kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the kinase domain of DAPK has a range of substrates that mediate its signalling, the additional protein interaction
Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subsequent neuronal damage
DeGregorio-RN, et al.
Redox Biology, 143-158 (2018)
DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity
Cohen O, et al.
The Embo Journal, 16(5), 998-1008 (1997)
DAPK1 mediates the G1 phase arrest in human nasopharyngeal carcinoma cells induced by grifolin, a potential antitumor natural product
Luo XJ, et al.
European Journal of Pharmacology, 670(2-3), 427-434 (2011)
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