MFE-296

98031101, human endometrium, Epithelial

• Synonyme(s) : MFE296 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: MFE-296, 98031101
• Source biologique: human endometrium
• Mode de croissance: Adherent
• Caryotype: Near Tetraploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Androgen receptor
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human caucasian endometrial adenocarcinoma

Description de la lignée cellulaire

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Application

Tumour biology studies, receptor analysis

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Milieu de culture

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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