C2C12 Cell Line from mouse

91031101, mouse muscle, Myoblast

• Synonyme(s) : C2-C12 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: C2C12 Cell Line from mouse, C3H muscle myoblast, 91031101
• Source biologique: mouse muscle
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Myoblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C3H muscle myoblast

Description de la lignée cellulaire

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Application

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Milieu de culture

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Autres remarques

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