RAG

89040605, mouse kidney, Ameboid-like

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: RAG, 89040605
• Source biologique: mouse kidney
• Mode de croissance: Adherent
• Caryotype: Modal no. 68, hypertriploid
• Morphologie: Ameboid-like
• Produits: ES-2
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse BALB/c renal adenocarcinoma

Description de la lignée cellulaire

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Application

Hybridisation, resistance and tumourigenicity studies

Milieu de culture

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Autres remarques

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