MCF7 Cell Line human

86012803, human breast (adenocarcinoma), Epithelial-like

• Synonyme(s) : IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• product name: MCF7 Cell Line human, from human breast(adenocarcinoma), 86012803
• Source biologique: human breast (adenocarcinoma)
• Mode de croissance: Adherent
• Caryotype: 2n = 46, hypertriploid to hypotetraploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Reported to express the oestrogen and progesterone receptor
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian breast adenocarcinoma

Description de la lignée cellulaire

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Application

Tumourigenicity and virus studies: Type B and C viruses

Profil d'ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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