Product 86012803, MCF7 Cell Line human, is not tested with RPMI. The recommended Culture Medium is EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
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A propos de cet article
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human breast (adenocarcinoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid to hypotetraploid
Morphology:
Epithelial-like
Manufacturer/tradename:
ECACC
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Laissez-nous vous aiderbiological source
human breast (adenocarcinoma)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 86012803-DNA-5UG, pkg of vial of cells 86012803-1VL
growth mode
Adherent
karyotype
2n = 46, hypertriploid to hypotetraploid
morphology
Epithelial-like
products
Not specified
receptors
Reported to express the oestrogen and progesterone receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Type B and C viruses
Biochem/physiol Actions
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Human Caucasian breast adenocarcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011440, human lung (lymphoblast), Lymphoblast | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88062428, human nerve, Not specified |
| biological source human breast (adenocarcinoma) | biological source human lung | biological source human lung (lymphoblast) | biological source human nerve |
| products Not specified | products Not specified | products Not specified | products Not specified |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| growth mode Adherent | growth mode Adherent | growth mode Suspension | growth mode Adherent |
| packaging pkg of vial of cells 86012803-1VL, tube of 5 μg 86012803-DNA-5UG | packaging pkg of vial of cells 10092309-1VL, tube of 5 μg 10092309-DNA-5UG | packaging tube of 5 μg 85011440-DNA-5UG, pkg of vial of cells 85011440-1VL | packaging pkg of vial of cells 88062428-1VL, tube of 5 μg 88062428-DNA-5UG |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
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Does this grow on RPMI? If so, what additional supplements does it need? If not, what recommended medium to be used to culture these cells?
1 answer-
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
1 answer-
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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