TKE2

11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.

• Synonyme(s) : TKE, TKE-2
• Code UNSPSC : 41106514

Propriétés

• product name: TKE2, from mouse(CD-1), female eye, 11033107
• Source biologique: eye; mouse (CD-1)
• Sexe: female
• Mode de croissance: Adherent
• Caryotype: Normal karyotype of the female mouse CD1 strain
• Morphologie: Epithelial; small and regular cobblestone-like shape
• Produits: Stains positive for nuclear p63 expression and K14 keratin.
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Mouse cornea epithelial progenitor cells

Description de la lignée cellulaire

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Profil d'ADN

Not specified

Milieu de culture

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 10² - 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Autres remarques

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