Mero-14

9100101, human lung, Spindle shaped cells

• Synonyme(s) : Mero14 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: Mero-14, 09100101
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: Number of chromosomes 72-75
• Morphologie: Spindle shaped cells
• Produits: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Description de la lignée cellulaire

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

Milieu de culture

Hams F10 + 15% FCS + 2mM Glutamine

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

Autres remarques

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