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C2488

Sigma-Aldrich

Citrate Buffer Solution, 0.09 M

Synonyme(s) :

Citric Acid Buffer Solution

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About This Item

Code UNSPSC :
12161700
eCl@ss :
32129211
Nomenclature NACRES :
NA.25

Description

Contains 1% chloroform
pH 4.8 at 25 °C.

Niveau de qualité

Forme

solution

Concentration

0.09 M

pH

4.8 (25 °C)

Application(s)

diagnostic assay manufacturing

Température de stockage

2-8°C

Description générale

Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues. In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis. With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.

Application

Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments. It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.

Autres remarques

Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.

Pictogrammes

Health hazard

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Carc. 2 - STOT RE 2 Oral

Organes cibles

Liver,Kidney

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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