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C1821

Sigma-Aldrich

Monoclonal Anti-Pan Cadherin antibody produced in mouse

clone CH-19, ascites fluid

Synonyme(s) :

Monoclonal Anti-Pan Cadherin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

CH-19, monoclonal

Poids mol.

antigen 135 kDa

Contient

15 mM sodium azide

Espèces réactives

canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections
indirect immunofluorescence: 1:500 using cultured MDBK cells
microarray: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

Application(s)

research pathology

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+ -dependent cell-cell adhesion. The N-terminal part of these molecule is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and α-actinin.
Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis.
Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared.
Neural-cadherin (N-cadherin) is a member of the classic cadherin family. It is mainly expressed in neural and mesenchymal tissues.

Spécificité

Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type.
Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin family. The cadherins migrate in the molecular weight range of 125 to 140 kDa and are tissue dependent. The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin′s-fixed and acetone-fixed tissue sections. It reacts with cultured cell line preparations. Cross-reaction has been observed with human, rabbit, bovine, goat, sheep, dog, pig, guinea pig, hamster, cat, rat, mouse, psammomys, chicken, frog and snake.

Immunogène

synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.

Application

Monoclonal Anti-Pan Cadherin antibody produced in mouse has been used in:
  • fluorescence imaging
  • immunohistochemistry
  • western blotting
  • immunoblotting
  • immunofluorescence

Monoclonal Anti-Pan Cadherin may be used for: immunochemical and immunocytochemical detection of members of the cadherin family in normal and neoplastic cells and tissues; identification of genetically engineered proteins containing the C-terminal cadherin tail; screening of cDNA expression libraries for identification of novel members of the cadherin family and demonstration of adherens-type cell-cell junctions irrespective of their cadherin subtype.

Actions biochimiques/physiologiques

Neural-cadherin (N-cadherin) has an oncogenic role in bladder cancer, breast cancer, prostate cancer and melanoma. It stimulates the heterotypic adhesion of melanoma cells to dermal fibroblast cells. N-cadherin acts as a critical modulator for the migration of neuronal progenitors. It is essential for the proper cohesion and polarisation of neuroepithelial cells. N-cadherin is important for normal gastrulation and neural crest development. It is required for myogenesis and myotube formation.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

N-cadherin promotes thyroid tumorigenesis through modulating major signaling pathways
Da C, et al.
Oncotarget, 8(5), 8131-8131 (2017)
Expression of cadherin adhesion molecules on human gametes
Rufas O, et al.
Molecular Human Reproduction, 6(2), 163-169 (2000)
N-cadherin and fibroblast growth factor receptors crosstalk in the control of developmental and cancer cell migrations
Nguyen T and Mege RM
European Journal of Cell Biology, 95(11), 415-426 (2016)
I M S Paulsen et al.
European journal of histochemistry : EJH, 59(4), 2532-2532 (2015-12-29)
Heat-induced epitope retrieval (HIER) is widely used for immunohistochemistry on formalin fixed paraffin-embedded tissue and includes temperatures well above the melting point of paraffin. We therefore tested whether traditional xylene-based removal of paraffin is required on sections from paraffin-embedded tissue
Sung Jun Bae et al.
eLife, 6 (2017-10-25)
The Hippo pathway controls tissue growth and homeostasis through a central MST-LATS kinase cascade. The scaffold protein SAV1 promotes the activation of this kinase cascade, but the molecular mechanisms remain unknown. Here, we discover SAV1-mediated inhibition of the PP2A complex

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