APPA014

HIV Protease Activity Detection Kit

• Synonyme(s) : Cut-N-Glow HIV Protease Detection Kit
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Forme: solution
• Niveau de qualité: 200
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.

Adéquation

This kit contains sufficient reagents for one 96 well plate (96 tests)

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable