A8887

Acrylamide

suitable for electrophoresis, ≥99%

• Synonyme(s) : 2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
• Formule linéaire : CH₂=CHCONH₂
• Numéro CAS: 79-06-1
• Poids moléculaire : 71.08
• Numéro Beilstein : 605349
• Numéro CE : 201-173-7
• Numéro MDL: MFCD00008032
• Code UNSPSC : 41105319
• eCl@ss : 39031205
• ID de substance PubChem : 24891378
• Nomenclature NACRES : NB.22

Propriétés

• Densité de vapeur: 2.45 (vs air)
• Niveau de qualité: 200
• Pression de vapeur: 0.03 mmHg ( 40 °C)
• Pureté: ≥99%
• Forme: crystals
• Technique(s): electrophoresis: suitable
• Point d'ébullition: 125 °C/25 mmHg (lit.)
• Pf: 82-86 °C (lit.)
• Solubilité: water: soluble 2.5 g/10 mL, clear, colorless
• Adéquation: suitable for electrophoresis
• Chaîne SMILES: NC(=O)C=C
• InChI: 1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
• Clé InChI: HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

Description générale

Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide has been used:

• for the synthesis of hydrogels
• for the preparation of polyacrylamide gel
• to agglomerate vimentin and keratin intermediate filament network

Acrylamide has been used:

• to agglomerate the vimentin and keratin intermediate filament network of K562cells
• in the preparation and characterization of soft culture substrate
• in hydrogel synthesis
• for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H301,H312 + H332,H315,H317,H319,H340,H350,H361f,H372
• Conseils de prudence: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Organes cibles: Peripheral nervous system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 280.4 °F - closed cup
• Point d'éclair (°C): 138 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges