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Key Documents

07679

Sigma-Aldrich

Abberior® STAR 635P, NHS ester

suitable for fluorescence

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About This Item

Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32

Poids mol.

Mw 1030 g/mol

Solubilité

DMF: 0.25 mg/mL, clear

Fluorescence

λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Description générale

Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.

Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.

Key Features
  • First phosphorylated dye
  • Unmatched, background free STED imaging contrast
  • Verified in the Leica STED system

Absorption Maximum, λmax: 638 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 120′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 - 780 nm
Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)

Adéquation

Designed and tested for fluorescent super-resolution microscopy

Informations légales

Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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