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Key Documents

L5910

Millipore

Lysine medium

suitable for microbiology, NutriSelect® Plus

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About This Item

Code UNSPSC :
41171606
Nomenclature NACRES :
NA.85

Stérilité

non-sterile

Niveau de qualité

Gamme de produits

BioChemika

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

pH final

5.0±0.2 (25 °C)

Application(s)

food and beverages
microbiology

Adéquation

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Application

Used for isolation and enumeration of wild yeasts in pitching yeasts.

Composants

Ingredients (g/L)
Dextrose, 44.50
Monopotassium phosphate, 1.78
Magnesium sulphate, 0.89
Calcium chloride, 0.178
Sodium chloride, 0.089
Adenine, 0.00178
DL-Methionine, 0.000891
L-Histidine, 0.000891
DL-Tryptophane, 0.000891
Boric acid, 0.0000089
Zinc sulphate, 0.0000356
Ammonium molybdate, 0.0000178
Manganese sulphate, 0.0000356
Ferrous sulphate, 0.0002225
L-Lysine, 1.00
Inositol, 0.02
Calcium pantothenate, 0.002
Aneurine, 0.0004
Pyridoxine, 0.0004
p-Aminobenzoic acid, 0.0002
Nicotinic acid, 0.0004
Riboflavin, 0.0002
Biotin, 0.000002
Folic acid, 0.000001
Agar, 17.80

Notes préparatoires

Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Morris, E.O., et al.
Journal of the Institute of Brewing, 63(1), 34-34 (1957)

Articles

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Protocoles

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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