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63052

Sigma-Aldrich

Magnesium acetate solution

BioUltra, for molecular biology, ~1 M in H2O

Synonyme(s) :

Magnesium acetate anhydrous, Mg(OAc)2

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About This Item

Formule linéaire :
(CH3COO)2Mg
Numéro CAS:
Poids moléculaire :
142.39
Numéro Beilstein :
3692530
Numéro MDL:
Code UNSPSC :
12161700
ID de substance PubChem :
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

Gamme de produits

BioUltra

Forme

liquid

Concentration

~1 M in H2O

Impuretés

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

Indice de réfraction

n20/D 1.358

pH

5.0-7.0 (25 °C)

Traces de cations

Al: ≤5 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mn: ≤5 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤2 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorption UV

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

Chaîne SMILES 

CC(=O)O[Mg]OC(C)=O

InChI

1S/2C2H4O2.Mg/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+2/p-2

Clé InChI

UEGPKNKPLBYCNK-UHFFFAOYSA-L

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Application

Magnesium acetate is used in molecular biology applications such as batch in vitro transcription of RNA and the crystallization of transcription factor:DNA complexes and proteins via the sitting-drop vapor-diffusion method.
Magnesium acetate solution has been used:
  • to supplement Midkin fused to the biotin ligase BioID2 (MK-BioID2) conditioned medium for in-tube biotinylation assay
  • as a component of lysis buffer and amplified RNA (aRNA) fragmentation buffer to perform aRNA fragmentation
  • as a component of lysis buffer and DamID buffer for sequencing zygote samples

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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Consulter la Bibliothèque de documents

Lena Haffo et al.
Scientific reports, 8(1), 12671-12671 (2018-08-25)
The tumor suppressor p53 is commonly inactivated in human tumors, allowing evasion of p53-dependent apoptosis and tumor progression. The small molecule APR-246 (PRIMA-1Met) can reactive mutant p53 in tumor cells and trigger cell death by apoptosis. The thioredoxin (Trx) and
Hina Akhter et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 203, 144-151 (2016-10-28)
The larvae of the mosquito, Aedes aegypti normally inhabit freshwater (FW) where they face dilution of body fluids by osmotic influx of water. In response, the physiological actions of the anal papillae result in ion uptake while the Malpighian tubules
Quentin M Dudley et al.
ACS synthetic biology, 5(12), 1578-1588 (2016-08-02)
Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor balance can be limiting. To address this
Roberto Stella et al.
Analytical and bioanalytical chemistry, 408(29), 8343-8353 (2016-10-04)
The illegal use of pharmacologically active compounds for growth promotion in food-producing species poses risks for consumer health and animal welfare. Surveillance relies on the quantification of drug residues in animal fluids or tissues, but the efficacy can be negatively
Cinthia R Millan et al.
Nucleic acids research, 45(14), 8378-8391 (2017-06-24)
Trypanosoma brucei, the causative agent of sleeping sickness (Human African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs. This has prompted studies of drugs interacting with AT-rich DNA, such as the N-phenylbenzamide

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