11179179001

Collagen

from rat tail tendon

• Synonyme(s) : collagen
• Code UNSPSC : 12352207

Propriétés

• Source biologique: rat tail
• Niveau de qualité: 100
• Stérilité: sterile
• Forme: lyophilized (clear, colorless solution after reconstitution)
• Conditionnement: pkg of 30 mg
• Fabricant/nom de marque: Roche
• Technique(s): cell culture | mammalian: suitable
• Numéro d'accès UniProt: P02454
• Température de stockage: 2-8°C
• Informations sur le gène: rat ... Col1a1(29393)

Description

Description générale

Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens  are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.

Spécificité

Active on most mammalian cells.

Application

Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.

Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.

Actions biochimiques/physiologiques

Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.

Caractéristiques et avantages

Contents
Lyophilizate, cell culture grade, 3 vials of 10mg

Caractéristiques

Biological activity: Tested for the promotion of adherence of HUV-EC cells.

Notes préparatoires

Working concentration: 5 μg/cm²
5 μg/cm² for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.

Reconstitution

IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.

Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash