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Key Documents

SCC169

Sigma-Aldrich

EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line

Mouse

Synonyme(s) :

JK1 Feeder Cells

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About This Item

Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.81

product name

EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line, Immortalized Mouse Feeder Cell Line

Source biologique

mouse

Fabricant/nom de marque

EmbryoMax®

Technique(s)

cell culture | stem cell: suitable

Conditions d'expédition

ambient

Description générale

JK1 is an immortalized CD34+SMA+ cell line derived from spontaneous transformation of C57/BL6 mouse testicular stromal cells. JK1 cells are able to support the long-term culture of a variety of primitive cells including multi-potent adult spermatogonial-derived stem cells, pluripotent murine embyronic stem cells and embyronic germ cells derived from primordial germ cells. Adult spermatogonial stem and progenitor cells (SPCs) cultured on JK1 feeder cells express key features of germ line stem cells including expression of PLZF, DAZL, and GCNA1. JK1 feeder cells has maintained its capacity for promoting stem cell self-renewal even after serial passaging for over one year.

Description de la lignée cellulaire

Fibroblast Cells

Application

Immortalized Mouse Feeder Cell Line
Research Category
Stem Cell Research
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Qualité

• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Stockage et stabilité

EmbryoMax JK1 Murine Testicular Stromal Feeder Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Informations légales

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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