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RMNPMAG-83K

Millipore

MILLIPLEX® Rat/Mouse Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay

The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84

Niveau de qualité

Espèces réactives

mouse, rat

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 107-135%
(Rat)

standard curve range: 14-100,000 pg/mL
(Oxytocin)

standard curve range: 27-20,000 pg/mL
(Neurotensin)

standard curve range: 3-2,000 pg/mL
(Substance P)

standard curve range: 41-30,000 pg/mL
(α-MSH)

standard curve range: 69-50,000 pg/mL
(β-Endorphine)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Description générale

The MILLIPLEX® Rat/Mouse Neuropeptide Bead Panel, containing 5 biomarkers. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate/culture supernatant samples and cerebrospinal fluid (CSF). Serum or plasma samples may also be used, and will need to be extracted, however we do not recommend using the panel for serum and plasma Substance P. The kit contains enough material to analyze 38 samples in duplicate, including the standard curve and two quality controls.

An important note about this panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.

The central nervous system is a complex environment consisting of billions of neurons as well as glial support cells. These neurons use many different chemical signals to communicate information, including neurotransmitters, cannabinoids, peptides, and gases such as nitric oxide. Neuropeptides are secreted primarily from the central and peripheral nervous systems, exerting a broad spectrum of biological functions that includes the regulation of metabolism, reproduction, and immunity.

Panel Type: Neuroscience

Application

  • Analytes: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.
  • Recommended Sample type: serum, plasma, CSF, and tissue culture
  • Recommended Sample dilution: This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture media per well
  • Assay Run Time: Overnight
  • NOTE: this is a competitive assay.

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Autres remarques

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Informations légales

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Skull and crossbonesEnvironment

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Willem J van den Brink et al.
The AAPS journal, 19(1), 274-285 (2016-10-28)
To reveal unknown and potentially important mechanisms of drug action, multi-biomarker discovery approaches are increasingly used. Time-course relationships between drug action and multi-biomarker profiles, however, are typically missing, while such relationships will provide increased insight in the underlying body processes.
Jen-Yin Goh et al.
Brain, behavior, and immunity, 89, 100-117 (2020-06-03)
Many psychiatric illnesses have a multifactorial etiology involving genetic and environmental risk factors that trigger persistent neurodevelopmental impairments. Several risk factors have been individually replicated in rodents, to understand disease mechanisms and evaluate novel treatments, particularly for poorly-managed negative and
Jorge Moreno-Fernández et al.
Nutrients, 11(10) (2019-10-09)
Iron deficiency anemia (IDA) is one of the most prevalent nutritional deficiencies worldwide. Iron plays critical roles in nervous system development and cognition. Despite the known detrimental consequences of IDA on cognition, available studies do not provide molecular mechanisms elucidating
Weiling Yin et al.
Neurobiology of aging, 83, 1-10 (2019-10-05)
The perimenopausal transition at middle age is often associated with hot flashes and sleep disruptions, metabolic changes, and other symptoms. Whereas the mechanisms for these processes are incompletely understood, both aging (AG) and a loss of ovarian estrogens play contributing
Germana Cocozza et al.
British journal of pharmacology, 178(24), 4891-4906 (2021-08-20)
Amyotrophic lateral sclerosis (ALS) patients exhibit dysfunctional energy metabolism and weight loss, which is negatively correlated with survival, together with neuroinflammation. However, the possible contribution of neuroinflammation to deregulations of feeding behaviour in ALS has not been studied in detail.

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