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PC712

Sigma-Aldrich

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-53BP1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Ne contient pas

preservative

Espèces réactives

mouse, human

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−70°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TP53(7157)

Description générale

Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).

Immunogène

Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence

Application

Immunoblotting (1:3000-1:5000)

Immunofluorescence (1:300-1:500, indirect)

Immunoprecipitation (1:300-1:500)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Remarque sur l'analyse

Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)

Autres remarques

Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Mariana Acevedo et al.
Cancer research, 76(11), 3252-3264 (2016-05-22)
Promyelocytic leukemia (PML) plays a tumor suppressive role by inducing cellular senescence in response to oncogenic stress. However, tumor cell lines fail to engage in complete senescence upon PML activation. In this study, we investigated the mechanisms underlying resistance to
Hiroshi Ikeuchi et al.
Oncogene (2024-08-03)
Identifying the mechanisms of action of anticancer drugs is an important step in the development of new drugs. In this study, we established a comprehensive screening platform consisting of 68 oncogenes (MANO panel), encompassing 243 genetic variants, to identify predictive
Mafuka Suzuki et al.
PloS one, 18(1), e0281168-e0281168 (2023-01-28)
Malignancy is often associated with therapeutic resistance and metastasis, usually arising after therapeutic treatment. These include radio- and chemo-therapies, which cause cancer cell death by inducing DNA double strand breaks (DSBs). However, it is still unclear how resistance to these
Haruka Fujimori et al.
Heliyon, 5(12), e03057-e03057 (2020-02-23)
Most cancers develop with one of two types of genomic instability, namely, chromosomal instability (CIN) or microsatellite instability (MSI). Both are induced by replication stress-associated DNA double-strand breaks (DSBs). The type of genomic instability that arises is dependent on the
Salma Akter et al.
Genes to cells : devoted to molecular & cellular mechanisms, 28(1), 53-67 (2022-11-24)
Steroid hormones induce the transcription of target genes by activating nuclear receptors. Early transcriptional response to various stimuli, including hormones, involves the active catalysis of topoisomerase II (TOP2) at transcription regulatory sequences. TOP2 untangles DNAs by transiently generating double-strand breaks

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