MABT1505

Anti-Plakophilin 1 Antibody, clone 10B2

clone 10B2, from mouse

• Synonyme(s) : Band 6 protein, B6
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 10B2, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunofluorescence: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_000290
• Numéro d'accès UniProt: Q13835
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PKP1(5317)

Description

Description générale

Plakophilin-1 (UniProt: Q13835; also known as Band 6 protein, B6P) is encoded by the PKP1 gene (Gene ID: 5317) in human. Plakophilins are proteins that are involved in linking adherins to cytoskeletal intermediate filaments. They contain numerous armadillo (ARM) repeats and localize to cell desmosomes and nuclei. Plakophilin-1 may be involved in molecular recruitment and stabilization during desmosome formation. It is also shown to be important for tooth development and regulates ZO-1 distribution in the dental epithelium. Two isoforms of Plankophilin-1 have been reported that are produced by alternative splicing. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia and isoform 2 is widely expressed. Plakophilin-1 is shown to be highly expressed in teeth and skin, and was upregulated during tooth development. It is reported to be involved in the regulation of Wnt signaling via its ARM repeat domains in a manner similar to beta-catenin and Wnt activation induces its nuclear translocation. Mutations n PKP1 gene are shown to cause ectodermal dysplasia-skin fragility syndrome that is characterized by cutaneous fragility and congenital ectodermal dysplasia affecting skin, hair and nails. (Ref.: Sprecher, E., et al (2004). J. Invest. Dermatol. 122(3); 647-51; Miyazaki, K et al. (2016). PLoS ONE 11(3): e0152206).

Spécificité

Clone 10B2 specifically detects Planophilin-1 and targets an epitope with in the first 27 amino acids from the N-terminal region.

Immunogène

Epitope: N-terminus

MBP-conjugated recombinant fragment corresponding to the first 235 amino acids from the N-terminal region of human Plakophilin-1.

Application

Anti-Plakophilin 1, clone 10B2, Cat. No. MABT1505, is a mouse monoclonal antibody that detects Plantophilin 1 and has been tested for use in Immunofluorescence and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected Plakophilin 1 in Western Blotting applications (Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).

Immunofluorescence Analysis: A representative lot detected Plakophilin 1 in Immunofluorescence applications (Sprecher, E., et. al. (2004). J Invest Dermatol. 122(3):647-51;Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).

Qualité

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin 1 in 10 µg of PC3 cell lysate.

Description de la cible

~80 kDa observed; 80.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash