MABN2635

Anti-Mitochondrial ferritin/FTMT Antibody, clone C65-2

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies, primary antibodies
• Clone: C65-2, monoclonal
• Poids mol.: calculated mol wt 27.54 kDa; observed mol wt ~29 kDa
• Produit purifié par: using protein G
• Espèces réactives: human, monkey
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgMκ
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_803431
• Numéro d'accès UniProt: Q8N4E7
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FTMT(94033)

Description

Description générale

Ferritin, mitochondrial (UniProt: Q8N4E7; also known as EC:1.16.3.1, FTMT) is encoded by the FTMT gene (Gene ID: 94033) in human. Mitochondria are shown to be the sites where iron is transformed into heme and Fe-S-clusters. Mitochondrial ferritin (FtMt), a novel H-type ferritin, with ferroxidase activity. It is a homooligomeric protein with 24 subunits that is synthesized with a transit peptide (aa 1-49) that is subsequently cleaved off in the mitochondrial matrix to produce the mature protein (aa 50-242). The functional molecule is roughly spherical and contains a central cavity into which the polymeric mineral iron core is deposited. It stores iron in a soluble, non-toxic, readily available form. It is detected in testis and erythroleukemia and is also expressed in very low levels in the brain and other tissues. FtMt levels appear to correlate more with mitochondrial abundance than with iron metabolism. Higher levels of FtMt are reported in the cerebral cortex of Alzheimer s disease patients and in the substantia nigra of subjects with Parkinson s disease. Although FtMt exhibits about 79% homology to H-chain ferritin (FTH), Clone C65-2 specifically recognize only the FtMt and does not react with FTH. (Ref.: Yang, M., et al. (2017). Acta Histochem. Cytochem. 50(1); 49-55; Yang, H., et al. (2013). Neurosci. Res. 77(1-2); 1-7).

Spécificité

Clone C65-2 is a mouse monoclonal antibody that detects mitochondrial ferritin. It targets an epitope within nine amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human mitochondrial ferritin.

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from HEK293 cells overexpressing Ferritin mitochondrial (FTMT).

Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Ferritin, mitochondrial (FTMT) in lysate from HEK293 cells overexpressing FTMT.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Mitochondrial ferritin/FTMT in Immunohistochemistry applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

Western Blotting Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Western Blotting applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

Immunofluorescence Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Immunofluorescence applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Mitochondrial ferritin/FTMT clone C65-2 , Cat. No. MABN2635, is a mouse monoclonal antibody that detects mitochondrial ferritin and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable