MABE1151

Anti-OASIS (CREB3L1) Antibody, clone 10H1

clone 10H1, from mouse

• Synonyme(s) : Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 10H1, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_443086
• Numéro d'accès UniProt: Q96BA8
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CREB3L1(90993)

Description

Description générale

Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in
unfolded protein response (UPR). It binds the DNA consensus sequence 5′-GTGXGCXGC-3′. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.

Spécificité

Clone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1).

Application

Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues.

Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).

Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).

Qualité

Evaluated by Immunohistochemistry in human prostate cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.

Description de la cible

57.00 kDa calculated.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable