MABC1236
Anti-DNA-PKcs Antibody, clone 3A9.1
clone 3A9.1, from mouse
Synonyme(s) :
DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
3A9.1, monoclonal
Espèces réactives
human
Technique(s)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PRKDC(5591)
Description générale
DNA-dependent protein kinase catalytic subunit (EC 2.7.11.1; UniProt P78527; also known as DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460) is encoded by the PRKDC (also known as HYRC, HYRC1, IMD26, XRCC7) gene (Gene ID 5591) in human. DNA-PK is a heterotrimer of PRKDC and the Ku p70/YRCC6-p86/XRCC5 dimer. It acts as a molecular sensor for DNA damage and is involved in DNA non-homologous end joining required for double-strand break (DSB) repair and V(D)J recombination. Upon binding to DNA, it expresses its catalytic properties. DNA-PKcs falls into the phosphatidylinositol (PI) 3-kinase family, however, biochemical assays indicate that it phosphorylates proteins and has no detectable activity toward lipids. DNA-PKcs is displays high similarity to PI kinase family members involved in cell cycle control, DNA repair, and DNA damage responses. Deficiency of DNA-PKcs is linked to severe combined immunodeficiency characterized by reduced or absent T and B cells, recurrent candidiasis, and lower respiratory tract infections. Ref.: Hartley, KO., et al. (1995). 82, 849-856.
Spécificité
Clone 3A9.1 detects DNA-PK catalytic subunit (DNA-PKcs), but not the regulatory subunit (Ku). Target epitope is present in both spliced isoforms of human DNA-PKcs reported by UniProt (P78527).
Immunogène
GST-tagged recombinant fragment from the region between FAT and KIP-binding domain of human DNA-PKcs
Application
Detect DNA-PK catalytic subunit (DNA-PKcs) using this mouse monoclonal Anti-DNA-PKcs Antibody, clone 3A9.1, Cat. No. MABC1236, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Qualité
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA-PKcs in 10 µg of K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA-PKcs in 10 µg of K562 cell lysate.
Description de la cible
~470 kDa observed. 469.1/466.5 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
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