MAB3612
Anti-BubR1 Antibody, clone 8G1
clone 8G1, Chemicon®, from mouse
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
8G1, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... BUB1B(701)
Description générale
The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. BubR1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.
Spécificité
Recognizes human BubR1.
Immunogène
Recombinant human BubR1.
Application
Detect BubR1 with Anti-BubR1 Antibody, clone 8G1 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.
Western blot using an ECL detection system. Reacts with the ~120 kDa BubR1 protein and higher bands due to hyperphosphorylations.
Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.
Optimal working dilutions must be determined by the end user.
Forme physique
Format: Purified
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry.
Burum-Auensen, E; De Angelis, PM; Schj?lberg, AR; Kravik, KL; Aure, M; Clausen, OP
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Linda Clijsters et al.
Cell cycle (Georgetown, Tex.), 13(15), 2370-2378 (2014-12-09)
Sister chromatid separation creates a sudden loss of tension on kinetochores, which could, in principle, re-activate the spindle checkpoint in anaphase. This so-called "anaphase problem" is probably avoided by timely inactivation of cyclin B1-Cdk1, which may prevent the spindle tension
David J Wynne et al.
Molecular biology of the cell, 27(22), 3395-3404 (2016-11-05)
The kinetochore is often depicted as having a disk-like architecture in which the outer layer of proteins, which engage microtubules and control checkpoint signaling, are built on a static inner layer directly linked to CENP-A chromatin. Here, applying three-dimensional (3D)
Kinetochore function is controlled by a phospho-dependent coexpansion of inner and outer components.
David J Wynne et al.
The Journal of cell biology, 210(6), 899-916 (2015-09-09)
It is widely accepted that the kinetochore is built on CENP-A-marked centromeric chromatin in a hierarchical order from inner to outer kinetochore. Recruitment of many kinetochore proteins depends on microtubule attachment status, but it remains unclear how their assembly/disassembly is
Erik Voets et al.
Biology open, 4(4), 484-495 (2015-03-10)
When cells enter mitosis, the anaphase-promoting complex/cyclosome (APC/C) is activated by phosphorylation and binding of Cdc20. The RXXL destruction box (D-box) of cyclin B1 only binds Cdc20 after release of the spindle checkpoint in metaphase, initiating cyclin B1 ubiquitination upon
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique