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MAB3516

Sigma-Aldrich

Anti-Heat Shock Protein 70 Antibody, clone W27

clone W27, Chemicon®, from mouse

Synonyme(s) :

HSP70

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

W27, monoclonal

Espèces réactives

bovine, human, monkey

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2a

Numéro d'accès NCBI

NM_002154.3
NM_198431.1

Numéro d'accès UniProt

P34932

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HSPA4(3308)

Spécificité

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.

Immunogène

HSP70 from HeLa cells.

Application

Immunoblotting: 0.5-1 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Forme physique

Format: Purified

Remarque sur l'analyse

Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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High dietary taurine reduces apoptosis and atherosclerosis in the left main coronary artery: association with reduced CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia.
Zulli, A; Lau, E; Wijaya, BP; Jin, X; Sutarga, K; Schwartz, GD; Learmont, J; Wookey et al.
Hypertension null
Chi Lam Au Yeung et al.
Nature communications, 7, 11150-11150 (2016-03-30)
Advanced ovarian cancer usually spreads to the visceral adipose tissue of the omentum. However, the omental stromal cell-derived molecular determinants that modulate ovarian cancer growth have not been characterized. Here, using next-generation sequencing technology, we identify significantly higher levels of
Elisa Teyssou et al.
Neurobiology of aging, 58, 239-239 (2017-07-19)
Mutations in UBQLN2 have been associated with rare cases of X-linked juvenile and adult forms of amyotrophic lateral sclerosis (ALS) and ALS linked to frontotemporal dementia (FTD). Here, we report 1 known (c.1489C>T, p.Pro497Ser, P497S) and 3 novel (c.1481C>T, p.Pro494Leu
A Franchi et al.
Journal of cellular biochemistry, 121(4), 2877-2888 (2019-11-07)
Cells can communicate with other neighboring or distant cells through the secretion of extracellular vesicles (EV), composed of a lipid bilayer and bearing surface molecules that allow them to recognize target cells. In this way, EV induce signaling via different
Jeanette D Walton et al.
Neoplasia (New York, N.Y.), 6(6), 838-845 (2005-02-22)
Cellular heterogeneity is a hallmark of human neuroblastoma tumors and cell lines. Within a single neuroblastoma are cells from distinct neural crest lineages whose relative abundance is significant for prognosis. We postulate that a self-renewing multipotent tumor stem cell, which
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