MAB1618-AF488

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor^™ 488 Conjugate

clone 74.1, from mouse, ALEXA FLUOR^™ 488

• Synonyme(s) : Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1, Cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosoli
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 74.1, monoclonal
• Espèces réactives: rat, Xenopus, fish, bovine, sheep, mouse, Drosophila, human
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG2b
• Numéro d'accès NCBI: NM_012144.2
• Numéro d'accès UniProt: Q0III3; Q29RQ3
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DYNC1I1(1780)

Description

Description générale

Cytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

Spécificité

Reacts with all known forms of the 74 kDa intermediate chain (IC) subunit of mammaliam cytoplasmic dynein. The antigenic site has been mapped to the N-terminal 60 amino acids, a region conserved in intermediate chains encoded by both the DYNC1I1 and DYNC1I2 genes.

Application

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor^™ 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cytosolic dynein intermediate chains in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.

Qualité

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cytosolic dynein intermediate chains in HeLa cells.

Description de la cible

~74 kDa observed.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable