MAB1087-I

Anti-Eosinophil Peroxidase Antibody, clone AHE-1

• Synonyme(s) : EPO, EPX
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: AHE-1, monoclonal
• Poids mol.: calculated mol wt 81.04 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_000493
• Numéro d'accès UniProt: P11678
• Température de stockage: 2-8°C
• Informations sur le gène: human ... EPX(8288)

Description

Description générale

Eosinophil peroxidase (UniProt: P11678; also known as EC:1.11.1.7, EPO, EPX) is encoded by the EPX (also known as EPER, EPO, EPP) gene (Gene ID: 8288) in human. Eosinophil peroxidase is an enzyme found in cytoplasmic granules of eosinophils. It mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. It is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-139), which are subsequently cleaved off to produce the mature form that can be cleaved into a light chain (aa 140-250) and a heavy chain (aa 251-715). These chains can assemble to for a tetrameric structure. It is a heme-containing enzyme that physically associates with fibrous tissue and cancer tissue in various organs. It contains well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional extracellular matrix (ECM) both in vitro and in vivo. It plays a role in regulating the recruitment of fibroblast and the biosynthesis of collagen ECM at sites of normal tissue repair and fibrosis. It is a key participant in generation of reactive oxidants and diffusible radical species by the phagocytes. It serves as a potent toxin towards invading parasites and the surrounding tissues. It also displays significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. (Ref.: DeNichilo, MO., et al. (2015). Am. J. Pathol. 185(5); 1372-1384; Mitra, SN., et al. (2000). Redox Rep. 5(4); 215-224).

Spécificité

Clone AHE-1 is a mouse monoclonal antibody that detects Eosinophil Peroxidase.

Immunogène

Human Eosinophils from a patient with hypereosinophilic syndrome.

Application

Quality Control Applications

Evaluated by Immunofluorescence in frozen Human colon tissue sections.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Eosinophil Peroxidase in frozen Human colon tissue sections.

Tested applications

Immunofluorescence Analysis: A representative lot detected Eosinophil Peroxidase in Immunofluorescence applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821).

Immunohistochemistry Applications: A representative lot detected Eosinophil Peroxidase in Immunohistochemistry applications (Spang, C., et al. (2017). J Musculoskelet Neuronal Interact. 17(3); 226-236; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821; Song, Y., et al. (2013). BMC Musculoskelet Disord. 14:; 34; Song, Y., et al. (2012). PLoS One ;7(12); e52230).

Immunocytochemistry Analysis: A representative lot detected Eosinophil Peroxidase in Immunocytochemistry applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable