Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

ABC131

Sigma-Aldrich

Anti-Ambra1 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse, human

Réactivité de l'espèce (prédite par homologie)

rat (based on 100% sequence homology)

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NP_7662587

Numéro d'accès UniProt

A2AH22

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... AMBRA1(55626)

Description générale

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) is a developmental protein that is involved in autophagy, differentiation and nuerogenesis. AMBRA1 regulates autophagy and development of the nervous system, normal cell survival and proliferation, and the control of protein turnover during neuronal development.

Spécificité

This antibody recognizes the C-terminus of Ambra1. This antibody should recognize isoforms 1 through 5 based upon immunogen design.

Immunogène

Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of mouse Ambra1.

Application

Anti-Ambra1 Antibody is a rabbit polyclonal antibody, validated for use in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 0.5 µg/mL of a representative lot detected Ambra1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of a representative lot detected Ambra1 in human cell lysates (HeLa & U87MG) and in mouse tissue homogenates (liver, kidney, and embryo), as well as exogenously expressed myc-Ambra1 in lysates from transfected HEK293 cells. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: Target specificity of a representative lot was confirmed by Western blotting analysis of Ambra1-knock mouse embryos and HeLa cells treated with Ambra1 siRNA. (Courtesy of Dr. Sabrina Di Bartolomeo, Cecconi Lab at the Dulbecco Telethon Institute, Italy).
Western Blotting Analysis: A representative lot detected decreased Ambra1 expression in tissue homogenates (kidney, liver, and lung) from Ambra1+/- mice when compared with tissue samples from wild-type mice. (Cianfanelli, V., et al. (2015). Nat. Cell Biol. 17(1):20-30).

Qualité

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ambra1 in 10 µg of MCF7 cell lysate.

Description de la cible

~140 kDa observed. Uniprot describes 7 isoforms at 143 kDa (isoform 1), 133 kDa (isoform 2), 87 kDa (isoform 3), 84 kDa (isoform 4), 100 kDa (isoform 5), 136 kDa (isoform 6), and 130kDa (Isoform 7). Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
MCF7 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Angelica Jardim Costa et al.
Pharmaceutics, 13(2) (2021-01-27)
The pharmacological modulation of autophagy is considered a promising neuroprotective strategy. While it has been postulated that lithium regulates this cellular process, the age-related effects have not been fully elucidated. Here, we evaluated lithium-mediated neuroprotective effects in young and aged
Matteo Tiberti et al.
Cell death & disease, 13(10), 872-872 (2022-10-16)
Cancer genomics and cancer mutation databases have made an available wealth of information about missense mutations found in cancer patient samples. Contextualizing by means of annotation and predicting the effect of amino acid change help identify which ones are more
Francesca Nazio et al.
Acta neuropathologica, 142(3), 537-564 (2021-07-25)
Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify
Manuela Antonioli et al.
Autophagy, 17(10), 2842-2855 (2020-11-12)
Oropharyngeal squamous cell carcinoma (OPSCC) is an increasing world health problem with a more favorable prognosis for patients with human papillomavirus (HPV)-positive tumors compared to those with HPV-negative OPSCC. How HPV confers a less aggressive phenotype, however, remains undefined. We
Alessandra Romagnoli et al.
Cell death & disease, 14(8), 505-505 (2023-08-06)
Mycobacterium tuberculosis (Mtb) is known to evade host immune responses and persist in macrophages for long periods. A mechanism that the host uses to combat Mtb is xenophagy, a selective form of autophagy that targets intracellular pathogens for degradation. Ubiquitination
Afficher tous les articles scientifiques apparentés

Articles

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.

Autophagy Mitophagy in Tumor Cell Survival Death

Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique